SARS-CoV-2 (COVID-19) Substrates



All substrates were designed using HyCoSuL technology (Hybrid Combinatorial Substrate Library, Poreba et al., Nature Protocols, 2017, (10): 2189-2214) in which natural and unnatural amino acids are used to optimize protease specificity of the tetrapeptide substrates. All substrates are linked to an ACC fluorophore due to its 2.8 -fold higher fluorescence quantum yield at the excitation and emission wavelengths of 380 nm and 460 nm. This results in higher sensitivity of the assay.



SARS-CoV-2-Mpro Substrate




Ac-Thz-Tle-Leu-Gln-ACC is a peptide substrate for both SARS-CoV-2 and SARS-CoV main protease, Mpro (also known as 3CLpro).

Molecular weight: 729.32, KM = 189.5 ± 2.7 µM, kcat = 0.144 ± 0.006 s-1, Kcat/KM = 760 ± 50 M-1s-1

Ref. Rut et al. https://www.biorxiv.org/content/10.1101/2020.03.07.981928v1.full-text






SARS-CoV-2-Mpro Substrate




Ac-Abu-Tle-Leu-Gln-ACC is a peptide substrate for both SARS-CoV-2 and SARS-CoV main protease, Mpro (also known as 3CLpro).

Molecular weight: 699.36, KM = 207.3 ± 12 µM, kcat = 0.178 ± 0.016 s-1, kcat/KM = 859 ± 57 M-1s-1.

Ref. Rut et al. https://www.biorxiv.org/content/10.1101/2020.03.07.981928v1.full-text






SARS-CoV-2-Mpro Substrate




Ac-Val-Lys-Leu-Gln-ACC is a peptide substrate for both SARS-CoV-2 and SARS-CoV main protease, Mpro (also known as 3CLpro).

Molecular weight: 728.39, KM = 228.4 ± 9.9 μM, kcat = 0.050 ± 0.002 s-1, kcat/KM = 219 ± 3 M-1 s-1

Ref. Rut et al. https://www.biorxiv.org/content/10.1101/2020.03.07.981928v1.full-text






SARS-CoV-2-PLpro Substrate




Ac-Leu-Arg-Gly-Gly-ACC is a peptide substrate for both SARS-CoV-2 and SARS-CoV Papain-Like cysteine protease, PLpro.

Molecular weight: 643.31, kcat/KM = 912 ± 49 M-1 s-1

Ref. Rut et al. https://www.biorxiv.org/content/10.1101/2020.04.29.068890v2









Substrate Ac-Thz-Tle-Leu-Gln-ACC 

 
Ac-Thz-Tle-Leu-Gln-ACC is a peptide substrate for both SARS-CoV-2 and SARS-CoV main protease, Mpro (also known as 3CLpro)
Species: N/A Source: N/A Expression: N/A Cell Line: N/A Sequence Type: N/A
Catalogue Number: CPW105.10500.10501




Substrate Ac-Abu-Tle-Leu-Gln-ACC

 
Ac-Abu-Tle-Leu-Gln-ACC  is a peptide substrate for both SARS-CoV-2 and SARS-CoV main protease, Mpro (also known as 3CLpro)
Species: N/A Source: N/A Expression: N/A Cell Line: N/A Sequence Type: N/A
Catalogue Number: CPW106.10502.10503




Substrate Ac-Val-Lys-Leu-Gln-ACC

 
Ac-Val-Lys-Leu-Gln-ACC  is a peptide substrate for both SARS-CoV-2 and SARS-CoV main protease, Mpro (also known as 3CLpro)
Species: N/A Source: N/A Expression: N/A Cell Line: N/A Sequence Type: N/A
Catalogue Number: CPW107.10504.10505




Substrate Ac-Leu-Arg-Gly-Gly-ACC  

 
Ac-Leu-Arg-Gly-Gly-ACC is a peptide substrate for both SARS-CoV-2 and SARS-CoV Papain-Like cysteine protease, PLpro
Species: N/A Source: N/A Expression: N/A Cell Line: N/A Sequence Type: N/A
Catalogue Number: CPW108.10506.10507
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