Proteases and Zymogens


All of the proteases involved in blood coagulation are trypsin-like members of the chymotrypsin family of serine proteases. They circulate as inactive zymogens, requiring cleavage of a conserved Arg15-Ile16 bond for activation of the protease domain. The new N-terminus at Ile16 becomes a tethered ligand, forming a salt-bridge with Asp 194 within a hydrophobic activation pocket. This event stabilises the substrate binding site and the oxyanion hole, conferring activity.

The zymogens and proteases in blood coagulation are mostly vitamin K factors, with N-terminal, membrane-anchoring gamma-carboxyglutamic acid (Gla) domains. These include: fVII, fIX, fX, prothrombin, protein C, and also the pseudo-protease cofactors, protein S and protein Z. With the exception of prothrombin, all of these factors are composed of an N-terminal Gla domain, two epidermal growth-factor-like (EGF) domains, and a C-terminal protease domain. Prothrombin has a Gla, two kringle domains, and a protease domain. Clotting proteases involved in contact activation (fIX, fXII, pre-kallikrein) are different with respect to domains and domain organisation, but these too possess a C-terminal chymotrypsin protease domain.

Factor X

  • Human factor X (plasma)

     
    Human factor X (plasma)
    Species: Human Source: Natural Expression: N/A Cell Line: N/A Sequence Type: Wild-type
  • Human factor X Gla-domainless (wild-type)

     
    Human beta-factor X EGF2 catalytic domain (wild-type) recombinant bacterial expression. Residues 86 to 435 consisting of the EGF2 domain activation peptide and protease domain.
    Species: Human Source: Recombinant Expression: Bacterial Cell Line: E coli Sequence Type: Wild-type
  • Human factor X Gla-domainless (S195A)

     
    Human beta-factor X EGF2 catalytic domain (S195A in chymotrypsin numbering, S379A in mature numbering) recombinant bacterial expression. Residues 86 to 435 consisting of the EGF2 domain activation peptide and protease domain. Serine 195 is the catalytic residue.
    Species: Human Source: Recombinant Expression: Bacterial Cell Line: E coli Sequence Type: Mutant
  • Human factor X (wild-type)

     
    Human factor X (wild-type) recombinant mammalian expression.
    Species: Human Source: Recombinant Expression: Mammalian Cell Line: HEK EBNA Sequence Type: Wild-type
  • Human factor X (S195A)

     
    Human factor X (S195A in chymotrypsin numbering, S379A in mature numbering) recombinant mammalian expression. Serine 195 is the catalytic residue.
    Species: Human Source: Recombinant Expression: Mammalian Cell Line: HEK EBNA Sequence Type: Mutant

Factor Xa

  • Human factor Xa (plasma)

     
    Human factor Xa (plasma)
    Species: Human Source: Natural Expression: N/A Cell Line: N/A Sequence Type: Wild-type
  • Human factor Xa Gla-domainless (wild-type)

     
    Human beta-factor Xa EGF2 catalytic domain (wild-type) recombinant bacterial expression. Residues 86 to 435 consisting of the EGF2 domain and protease domain.
    Species: Human Source: Recombinant Expression: Bacterial Cell Line: E coli Sequence Type: Wild-type
  • Human factor Xa Gla-domainless (S195A)

     
    Human beta-factor Xa EGF2 catalytic domain (S195A in chymotrypsin numbering, S379A in mature numbering) recombinant bacterial expression. Residues 86 to 435 consisting of the EGF2 domain and protease domain. Serine 195 is the catalytic residue.
    Species: Human Source: Recombinant Expression: Bacterial Cell Line: E coli Sequence Type: Mutant
  • Human polyhistadine-tagged factor Xa

     
    Human polyhistadine-tagged factor Xa recombinant bacterial expression. The polyhistadine-tag allows the easy removal of factor Xa from a cleavage reaction on a nickle column.
    Species: Human Source: Recombinant Expression: Bacterial Cell Line: E coli Sequence Type: Mutant
  • Human factor Xa (wild-type)

     
    Human factor Xa (wild-type) recombinant mammalian expression.
    Species: Human Source: Recombinant Expression: Mammalian Cell Line: HEK EBNA Sequence Type: Wild-type
  • Human factor Xa (S195A)

     
    Human factor Xa (S195A in chymotrypsin numbering, S379A in mature numbering) recombinant mammalian expression. Serine 195 is the catalytic residue.
    Species: Human Source: Recombinant Expression: Mammalian Cell Line: HEK EBNA Sequence Type: Mutant

Factor IX

  • Human factor IX (plasma)

     
    Human factor IX (plasma)
    Species: Human Source: Natural Expression: N/A Cell Line: N/A Sequence Type: Wild-type
  • Human factor IX Gla-domainless (wild-type)

     
    Human factor IX EGF2 catalytic domain (wild-type) recombinant bacterial expression. Residues 85 to 415 consisting of the EGF2 domain activation peptide and protease domain.
    Species: Human Source: Recombinant Expression: Bacterial Cell Line: E coli Sequence Type: Wild-type
  • Human factor IX Gla-domainless (S195A)

     
    Human factor IX EGF2 catalytic domain (S195A in chymotrypsin numbering, S365A in mature numbering) recombinant bacterial expression. Residues 85 to 415 consisting of the EGF2 domain activation peptide and protease domain. Serine 195 is the catalytic residue.
    Species: Human Source: Recombinant Expression: Bacterial Cell Line: E coli Sequence Type: Mutant

Factor IXa

  • Human factor IXa (plasma)

     
    Human factor IXa (plasma)
    Species: Human Source: Natural Expression: N/A Cell Line: N/A Sequence Type: Wild-type
  • Human factor IXa Gla-domainless (wild-type)

     
    Human factor IXa EGF2 catalytic domain (wild-type) recombinant bacterial expression. Residues 85 to 415 consisting of the EGF2 domain and protease domain.
    Species: Human Source: Recombinant Expression: Bacterial Cell Line: E coli Sequence Type: Wild-type
  • Human factor IXa Gla-domainless (S195A)

     
    Human factor IXa EGF2 catalytic domain (S195A in chymotrypsin numbering, S365A in mature numbering) recombinant bacterial expression. Residues 85 to 415 consisting of the EGF2 domain and protease domain. Serine 195 is the catalytic residue.
    Species: Human Source: Recombinant Expression: Bacterial Cell Line: E coli Sequence Type: Mutant
  • Human factor IXa Gla-domainless (wild-type) EGR-CK inhibited

     
    Human factor IXa Gla-domainless (wild-type) Glu-Gly-Arg-chloromethylketone inhibited recombinant bacterial expression.
    Species: Human Source: Recombinant Expression: Bacterial Cell Line: E coli Sequence Type: Wild-type

Protein C

  • Human protein C (plasma)

     
    Human protein C (plasma)
    Species: Human Source: Natural Expression: N/A Cell Line: N/A Sequence Type: Wild-type
  • Human protein C Gla-domainless (wild-type)

     
    Human protein C EGF2 catalytic domain (wild-type) recombinant mammalian expression.
    Species: Human Source: Recombinant Expression: Mammalian Cell Line: HEK EBNA Sequence Type: Wild-type
  • Human protein C Gla-domainless (S195A)

     
    Human protein C EGF2 catalytic domain (S195A in chymotrypsin numbering, S360A in mature numbering) recombinant mammalian expression. Serine 195 is the catalytic residue.
    Species: Human Source: Recombinant Expression: Mammalian Cell Line: HEK EBNA Sequence Type: Mutant
  • Human activated protein C (plasma)

     
    Human activated protein C (plasma)
    Species: Human Source: Natural Expression: N/A Cell Line: N/A Sequence Type: Wild-type
  • Human activated protein C Gla-domainless (wild-type)

     
    Human activated protein C EGF2 catalytic domain (wild-type) recombinant mammalian expression
    Species: Human Source: Recombinant Expression: Mammalian Cell Line: HEK EBNA Sequence Type: Wild-type
  • Human activated protein C Gla-domainless (S195A)

     
    Human activated protein C EGF2 catalytic domain (S195A in chymotrypsin numbering, S360A in mature numbering) recombinant mammalian expression. Serine 195 is the catalytic residue.
    Species: Human Source: Recombinant Expression: Mammalian Cell Line: HEK EBNA Sequence Type: Mutant
  • Murine activated protein C Gla-domainless (wild-type)

     
    Murine activated protein C Gla-domainless (wild-type) recombinant mammalian expression (mouse).
    Species: Murine Source: Recombinant Expression: Mammalian Cell Line: HEK EBNA Sequence Type: Wild-type
  • Rat activated protein C Gla-domainless (wild-type)

     
    Rat activated protein C Gla-domainless (wild-type) recombinant mammalian expression.
    Species: Rat Source: Recombinant Expression: Mammalian Cell Line: HEK EBNA Sequence Type: Wild-type
  • Canine activated protein C Gla-domainless (wild-type)

     
    Canine activated protein C Gla-domainless (wild-type) recombinant mammalian expression.
    Species: Canine Source: Recombinant Expression: Mammalian Cell Line: HEK EBNA Sequence Type: Wild-type

Factor XI